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KMID : 0881720130280030247
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Journal of Food Hygiene and Safety
2013 Volume.28 No. 3 p.247 ~ p.251
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Simultaneous Determination of Non-steroidal Anti-inflammatory Drugs and Corticosteroids Added to Foods as Adulterants using LC-ESI-tandem Mass Spectrometry
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Lee Yong-Chul
Park Ju-Sung
Kim Sung-Dan
Yang Hye-Ran
Kim Eun-Hee
Lee Yun-Jeong
Jo Sung-Ja
Jo Han-Bin
Kim Jung-Hun
Chae Young-Zoo
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Abstract
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The objective of present study was to develop a simultaneous determination method of 5 medical compounds, including beclomethasone, dexamethasone, prednisolone, ketoprofen, phenylbutazone in foods, using LC-MS/MS. To optimize MS analytical condition of 5 compounds, each parameter was established by MRM mode. The chromatographic separation was achieved on a C18 column successfully, with a mobile phase made up of A (0.1% formic acid) and B (0.1% formic acid in acetonitrile), at a flow rate of 0.3 mL/min for 17 min with a gradient elution. LOD and LOQ of 5 compounds were in the range of 0.40~4.60 ng/mL and 0.81~11.46 ng/mL, respectively. As a result of analyzing the three concentrations of the standard mixture added to blank samples, the results showed that the mean recovery rate of 5 compounds was in the range of 81.52~103.83%, and RSD (%) of Intra- and Inter-day assay were 0.52-10.45. Since relatively fine selectivity, accuracy and reproducibility were shown in this qualified experimental method, it could be utilized efficiently to investigating those 5 compounds to see if it is added to food products illegally.
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KEYWORD
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ketoprofen, phenylbutazone, dexamethasone, prednisolone, LC-MS/MS
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